Scanning Electron Microscopy Observation of Adhesion Properties of Bifidobacterium longum W11 and Chromatographic Analysis of Its Exopolysaccaride
نویسندگان
چکیده
Bifidobacterium spp. can produce cell-bound or released exopolysaccharide (EPS) that is a beneficial trait mediating commensal-host interactions. However differences in the physico-chemical characteristics of EPS produced by different strains of Bifidobacterium spp. are determinant in adhesion ability and modulation of immune response. The aim of this study was to investigate the in vitro adhesion characteristics of Bifidobacterium longum W11 to intestinal epithelial cell-line HT-29, by Scanning Electron Microscopy (SEM), and chemical characteristics of its exopolysaccharide using Thin-Layer Chromatography (TLC) analysis. SEM observation showed a good adhesion of B. longum W11 to the HT-29 monolayer that could be increased by the production of exocellular polymers formation. TLC analysis of the purified and hydrolyzed EPS showed that the cell-surface and extracellular polysaccharide were composed mainly of fructose and glucose. Moreover, other sugars were present in smaller quantities. Information from this study on physico-chemical characteristics of EPS of B. longum W11 could contribute to understanding the physiology of bifidobacteria and their interaction with the host. Scanning Electron Microscopy observation of adhesion properties of Bifidobacterium longum W11 and chromatographic analysis of its exopolysaccaride. Corresponding author.
منابع مشابه
Complete Genome Sequence of Bifidobacterium longum W11 (LMG P-21586), Used as a Probiotic Strain
We report the complete genome sequence of Bifidobacterium longum W11 (LMG P-21586) isolated from the intestinal microbiota of a healthy man. The analysis of the sequence may provide insights into the microbiological characteristics and the functional activity of this probiotic strain.
متن کاملAdhesion properties of a putative polymorphic fimbrial subunit protein from Bifidobacterium longum subsp. longum
In our previous study, we found that the open reading frame bl0675 in the genome of Bifidobacterium longum subsp. longum isolated from human feces encoded a novel putative fimbrial protein, was highly polymorphic, and had five variants (A, B, C, D, and E types). The aim of this study was to evaluate the affinity of these variants to porcine colonic mucins (PCMs). Protein-binding properties were...
متن کاملIdentification of Bifidobacterium Strains Isolated from Fecal Samples of Some Iranian Subjects Using 16SrRNA Gene Sequence Analysis and PCR-based Gene Specific Primers
For the first time in Iran 40 strains of Bifidobacterium were isolated from feces of Iranian subjects. By using phenotypic tests, 18 isolates were identified as Bifidobacterium longum, 10 as Bifidobacterium bifidum and one as Bifidobacterium catenolatum. In order to validate these results and also to identify other isolates that had not been identified by phenotypic tests, two methods of PCR wi...
متن کاملEffect of silica particles on adhesion strength of polyvinyl chloride coatings on metal substrates
The aim of this study was to improve the adhesion performance of plasticized polyvinyl chloride (PVC) coatings on steel substrates by using nanoparticles. For this purpose, the PVC plastisol with different concentration of nano-silica was prepared and applied to bond steel joints. The adhesive strength of the joints was determined by single-lap shear test. Moreover, mechanical properties and mi...
متن کاملPhenotypic and Genotypic Characterization of Bifidobacterium Isolates from Healthy Adult Koreans
A total of twenty-two strict anaerobic and Gram-positive Bifidobacteria, identified as B. adolescentis, B.pseudocatenulatum, or B. longum, were isolated from healthy adult Koreans. We here investigated the cellmorphology, antimicrobial resistance patterns to novel antibiotics and genotypic differentiation ofBifidobacteria assessing repetitive DNA element PCR (rep-PCR) fingerpr...
متن کامل